Email:
p.kougias@elgo.gr
Academic Profiles
Dr. Panagiotis Kougias is a Senior Researcher at the Institute of Soil and Water Resources of the Hellenic Agricultural Organization DIMITRA. He holds a Bachelor and M.Eng. degree in Agriculture (specialization in Land Reclamation, Soil Science and Agricultural Engineering, 2005), an MSc (2007) and a PhD (2011) in Agricultural Engineering from the School of Agriculture of Aristotle University of Thessaloniki. He has previously served as research staff at the same School and spent a decade at the Technical University of Denmark, working as a postdoctoral researcher, researcher, senior researcher and Associate Professor, gaining extensive international experience in environmental biotechnology and bioprocess engineering. His research interests focus on the treatment of agro-industrial waste, the design and optimization of bioreactors, anaerobic bioprocesses, biogas production and upgrading, recovery of high value-added products and environmental microbiology. He has authored more than 150 scientific publications (including over 112 papers in ISI journals, H-index 47) and has supervised 7 PhD candidates and more than 12 MSc students. Since 2020 he has been listed among the world’s top 2% scientists, while he has received IKY scholarships for his MSc and PhD studies and has extensive experience as principal investigator, coordinator or work package leader in competitive national and European projects (GSRT, HFRI, ERANET, LIFE, Horizon Europe).
Kalaitzidis, A., Mitraka, G.-C., Katsantonis, D., Prasad, S., Singh, A., Koutroubas, S., Kougias, P. G., & Korres, N. E. (2026). Enhancing methane production from rice crop residues via pretreatment and co-digestion with cattle or swine slurry. Frontiers in Energy Research, 13. https://doi.org/10.3389/fenrg.2025.1680055
Xirostylidou, A., Kontogiannopoulos, K. N., Chatzis, A., Samaras, P., Zouboulis, A. I., & Kougias, P. G. (2026). Enhancing biogenic carbon dioxide conversion to acetic acid using support materials and machine learning-based optimization. Bioresource Technology, 441, 133485. https://doi.org/10.1016/j.biortech.2025.133485
Anagnostopoulou, C., Vargas-Estrada, L., Kougias, P. G., & Muñoz, R. (2026). Boosting Arthrospira platensis metabolism during biogas upgrading via carbon coated iron-based nanoparticle addition. Process Biochemistry, 162, 32–42. https://doi.org/10.1016/j.procbio.2026.01.004
Theodoropoulou, A., Bagaki, D. A., Gaspari, M., Kougias, P., Treu, L., Campanaro, S., Hidalgo, D., Timmers, R. A., Zrimec, M. B., Reinhardt, R., Grimalt-Alemany, A., Goonesekera, E. M., Angelidaki, I., Vasilaki, V., Malamis, D., Barampouti, E. M., & Mai, S. (2025). Technological Approaches for the Capture and Reuse of Biogenic Carbon Dioxide Towards Sustainable Anaerobic Digestion. Sustainability (Switzerland), 17(22). https://doi.org/10.3390/su172210385
Chatzis, A., Gaspari, M., Kontogiannopoulos, K. N., Sanguineti, D., Zampieri, G., Zouboulis, A. I., & Kougias, P. G. (2025). Assessment of the performance of packing materials in trickle bed reactors for efficient biomethanation. Journal of Environmental Chemical Engineering, 13(6). https://doi.org/10.1016/j.jece.2025.119480
Ghiotto, G., Xirostylidou, A., Gaspari, M., Kougias, P. G., Campanaro, S., & Treu, L. (2025). Exploring genetic adaptation and microbial dynamics in engineered anaerobic ecosystems via strain-level metagenomics. Cell Genomics, 5(9). https://doi.org/10.1016/j.xgen.2025.100949
Mola, M., Stratilaki, E., Mourouzidou, S., Kougias, P. G., Statiris, E., Papatheodorou, E. M., Malamis, S., & Monokrousos, N. (2025). Seasonal dynamics and functional diversity of soil nematode communities under treated wastewater irrigation in abandoned agricultural soils. Journal of Environmental Management, 375. https://doi.org/10.1016/j.jenvman.2025.124231
He, X., Peng, Z., Shen, P., Miao, C., Xie, J., Deng, C., Kougias, P. G., Shen, D., & Lin, R. (2025). Promoting Multi-Carbon Fatty Acids Production from Microbial Chain Elongation of CO2/H2 with Carbonaceous Materials. ACS Sustainable Chemistry and Engineering, 13(18), 6720–6734. https://doi.org/10.1021/acssuschemeng.5c01443
Ghiotto, G., De Bernardini, N., Orellana, E., Fiorito, G., Cenci, L., Kougias, P. G., Campanaro, S., & Treu, L. (2025). Impact of trace metal supplementation on anaerobic biological methanation under hydrogen and carbon dioxide starvation. Npj Biofilms and Microbiomes, 11(1). https://doi.org/10.1038/s41522-025-00649-2
Christianides, D., Bagaki, D. A., Timmers, R. A., Zrimec, M. B., Theodoropoulou, A., Angelidaki, I., Kougias, P., Zampieri, G., Kamergi, N., Napoli, A., Malamis, D., Mai, S., & Barampouti, E. M. (2025). Biogenic CO2 Emissions in the EU Biofuel and Bioenergy Sector: Mapping Sources, Regional Trends, and Pathways for Capture and Utilisation. Energies, 18(6). https://doi.org/10.3390/en18061345
Sanguineti, D., Chatzis, A., Zampieri, G., Gaspari, M., Kougias, P. G., Campanaro, S., & Treu, L. (2025). Modelling microbial and metabolic shifts in trickle bed reactor biomethanation at decreasing gas retention times. Chemical Engineering Journal, 522. https://doi.org/10.1016/j.cej.2025.167574
Chatzis, A., Kontogiannopoulos, K. N., Dimitrakakis, N., Zouboulis, A., & Kougias, P. G. (2025). Harnessing the Influence of Pressure and Nutrients on Biological CO2 Methanation Using Response Surface Methodology and Artificial Neural Network—Genetic Algorithm Approaches. Fermentation, 11(1). https://doi.org/10.3390/fermentation11010043
Ghiotto, G., De Bernardini, N., Giangeri, G., Tsapekos, P., Gaspari, M., Kougias, P. G., Campanaro, S., Angelidaki, I., & Treu, L. (2024). From microbial heterogeneity to evolutionary insights: A strain-resolved metagenomic study of H2S-induced changes in anaerobic biofilms. Chemical Engineering Journal, 485. https://doi.org/10.1016/j.cej.2024.149824
Xirostylidou, A., Gaspari, M., Kontogiannopoulos, K. N., Ghiotto, G., Treu, L., Campanaro, S., Zouboulis, A. I., & Kougias, P. G. (2024). Biomethanation on demand: Continuous and intermittent hydrogen supply on biological CO2 methanation. Chemical Engineering Journal, 495. https://doi.org/10.1016/j.cej.2024.153677
Agostini, S., Bucci, L., Doni, D., Costantini, P., Gupte, A., Müller, B., Sibilla, F., Basaglia, M., Casella, S., Kougias, P. G., Campanaro, S., Favaro, L., & Treu, L. (2024). Bioaugmentation strategies based on bacterial and methanogenic cultures to relieve stress in anaerobic digestion of protein-rich substrates. Renewable Energy, 225. https://doi.org/10.1016/j.renene.2024.120270
Nikolaidou, C., Mola, M., Papakostas, S., Aschonitis, V. G., Monokrousos, N., & Kougias, P. G. (2024). The effect of anaerobic digestate as an organic soil fertilizer on the diversity and structure of the indigenous soil microbial and nematode communities. Environmental Science and Pollution Research. https://doi.org/10.1007/s11356-024-32850-9
Mitraka, G.-C., Kontogiannopoulos, K. N., Zouboulis, A. I., & Kougias, P. G. (2024). Evaluation of the optimal sewage sludge pre-treatment technology through continuous reactor operation: Process performance and microbial community insights. Water Research, 257. https://doi.org/10.1016/j.watres.2024.121662
Proskynitopoulou, V., Vourros, A., Garagounis, I., Toursidis, P. D., Lorentzou, S., Kougias, P., Zouboulis, A., & Panopoulos, K. D. (2024). Treatment of anaerobically digested pig manure by applying membrane processes for nutrient recovery and antibiotics removal. Environmental Science and Pollution Research. https://doi.org/10.1007/s11356-024-33313-x
Anagnostopoulou, C., Papachristou, I., Kontogiannopoulos, K. N., Mourtzinos, I., & Kougias, P. G. (2024). Optimization of microalgae cultivation in food industry wastewater using microplates. Sustainable Chemistry and Pharmacy, 39. https://doi.org/10.1016/j.scp.2024.101510
Kappa, S., Nikolaidou, C., Noutsopoulos, C., Mamais, D., Hadjimitsi, E., Kougias, P. G., & Malamis, S. (2024). Investigating upflow anaerobic sludge blanket process to treat forward osmosis effluents of concentrated municipal wastewater under psychrophilic temperature. Bioresource Technology, 412. https://doi.org/10.1016/j.biortech.2024.131361
Mola, M., Kougias, P. G., Statiris, E., Papadopoulou, P., Malamis, S., & Monokrousos, N. (2024). Short-term effect of reclaimed water irrigation on soil health, plant growth and the composition of soil microbial communities. Science of the Total Environment, 949. https://doi.org/10.1016/j.scitotenv.2024.175107
Giangeri, G., Tsapekos, P., Gaspari, M., Ghofrani-Isfahani, P., Treu, L., Kougias, P., Campanaro, S., & Angelidaki, I. (2024). A bioaugmentation strategy to recover methane production under sulfate-stressed conditions: Highlights on targeted sulfate-reducing bacteria and DIET-related species. Applied Energy, 362. https://doi.org/10.1016/j.apenergy.2024.122940
Gaspari, M., Ghiotto, G., Centurion, V. B., Kotsopoulos, T., Santinello, D., Campanaro, S., Treu, L., & Kougias, P. G. (2024). Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics. Environmental Science and Technology, 58(1), 591–602. https://doi.org/10.1021/acs.est.3c07840
Anagnostopoulou, C., Papachristou, I., Kyriakoudi, A., Kontogiannopoulos, K. N., Mourtzinos, I., & Kougias, P. G. (2024). Development of alginate beads loaded with bioactive ingredients from Chlorella vulgaris cultivated in food industry wastewaters. Algal Research, 80. https://doi.org/10.1016/j.algal.2024.103530
Gkotsis, P., Kougias, P., Mitrakas, M., & Zouboulis, A. (2023). Biogas upgrading technologies – Recent advances in membrane-based processes. International Journal of Hydrogen Energy, 48(10), 3965–3993. https://doi.org/10.1016/j.ijhydene.2022.10.228
Chatzis, A., Orellana, E., Gaspari, M., Kontogiannopoulos, K., Treu, L., Zouboulis, A., & Kougias, P. G. (2023). Comparative study on packing materials for improved biological methanation in trickle Bed reactors. Bioresource Technology, 385. https://doi.org/10.1016/j.biortech.2023.129456
Simeonidis, K., Martinez-Boubeta, C., Kellartzis, I., Makridis, A., Delli, E., Haeussler, A., Kougias, P. G., Vourlias, G., & Balcells, L. (2023). Solar-assisted approach for the synthesis of nanoadsorbents for biogas desulfurization using wastes. Materials Today Energy, 37. https://doi.org/10.1016/j.mtener.2023.101395
Giangeri, G., Tsapekos, P., Gaspari, M., Ghofrani-Isfahani, P., Lin, M. K. T. H., Treu, L., Kougias, P., Campanaro, S., & Angelidaki, I. (2023). Magnetite Alters the Metabolic Interaction between Methanogens and Sulfate-Reducing Bacteria. Environmental Science and Technology, 57(43), 16399–16413. https://doi.org/10.1021/acs.est.3c05948
Gaspari, M., Alvarado-Morales, M., Tsapekos, P., Angelidaki, I., & Kougias, P. (2023). Simulating the performance of biogas reactors co-digesting ammonia and/or fatty acid rich substrates. Biochemical Engineering Journal, 190. https://doi.org/10.1016/j.bej.2022.108741
Gaspari, M., Treu, L., Centurion, V. B., Kotsopoulos, T. Α., Campanaro, S., & Kougias, P. G. (2023). Impacts of long chain fatty acids injection on biogas reactors performance stability and microbial community structure and function. Journal of Cleaner Production, 418. https://doi.org/10.1016/j.jclepro.2023.138048
Tsigkou, K., Terpou, A., Treu, L., Kougias, P. G., & Kornaros, M. (2022). Thermophilic anaerobic digestion of olive mill wastewater in an upflow packed bed reactor: Evaluation of 16S rRNA amplicon sequencing for microbial analysis. Journal of Environmental Management, 301. https://doi.org/10.1016/j.jenvman.2021.113853
Mitraka, G.-C., Kontogiannopoulos, K. N., Tsivintzelis, I., Zouboulis, A. I., & Kougias, P. G. (2022). Optimization of supercritical carbon dioxide explosion for sewage sludge pre-treatment using response surface methodology. Chemosphere, 297. https://doi.org/10.1016/j.chemosphere.2022.133989
Mitraka, G.-C., Kontogiannopoulos, K. N., Batsioula, M., Banias, G. F., Zouboulis, A. I., & Kougias, P. G. (2022). A Comprehensive Review on Pretreatment Methods for Enhanced Biogas Production from Sewage Sludge. Energies, 15(18). https://doi.org/10.3390/en15186536
Ghofrani-Isfahani, P., Tsapekos, P., Peprah, M., Kougias, P., Zervas, A., Zhu, X., Yang, Z., Jacobsen, C. S., & Angelidaki, I. (2022). Ex-situ biogas upgrading in thermophilic trickle bed reactors packed with micro-porous packing materials. Chemosphere, 296. https://doi.org/10.1016/j.chemosphere.2022.133987
Palù, M., Peprah, M., Tsapekos, P., Kougias, P., Campanaro, S., Angelidaki, I., & Treu, L. (2022). In-situ biogas upgrading assisted by bioaugmentation with hydrogenotrophic methanogens during mesophilic and thermophilic co-digestion. Bioresource Technology, 348. https://doi.org/10.1016/j.biortech.2022.126754
Tsapekos, P., Khoshnevisan, B., Zhu, X., Treu, L., Alfaro, N., Kougias, P. G., & Angelidaki, I. (2022). Lab- and pilot-scale anaerobic digestion of municipal bio-waste and potential of digestate for biogas upgrading sustained by microbial analysis. Renewable Energy, 201, 344–353. https://doi.org/10.1016/j.renene.2022.10.116
Anagnostopoulou, C., Kontogiannopoulos, K. N., Gaspari, M., Morlino, M. S., Assimopoulou, A. N., & Kougias, P. G. (2022). Valorization of household food wastes to lactic acid production: A response surface methodology approach to optimize fermentation process. Chemosphere, 296. https://doi.org/10.1016/j.chemosphere.2022.133871
Rossi, A., Morlino, M. S., Gaspari, M., Basile, A., Kougias, P., Treu, L., & Campanaro, S. (2022). Analysis of the anaerobic digestion metagenome under environmental stresses stimulating prophage induction. Microbiome, 10(1). https://doi.org/10.1186/s40168-022-01316-w
De Bernardini, N., Basile, A., Zampieri, G., Kovalovszki, A., De Diego Diaz, B., Offer, E., Wongfaed, N., Angelidaki, I., Kougias, P. G., Campanaro, S., & Treu, L. (2022). Integrating metagenomic binning with flux balance analysis to unravel syntrophies in anaerobic CO2 methanation. Microbiome, 10(1). https://doi.org/10.1186/s40168-022-01311-1
Abbaszadeh, L., Koutra, E., Tsigkou, K., Gaspari, M., Kougias, P. G., & Kornaros, M. (2022). Nitrification upon Nitrogen Starvation and Recovery: Effect of Stress Period, Substrate Concentration and pH on Ammonia Oxidizers’ Performance. Fermentation, 8(8). https://doi.org/10.3390/fermentation8080387
Khoshnevisan, B., He, L., Xu, M., Valverde-Pérez, B., Sillman, J., Mitraka, G.-C., Kougias, P. G., Zhang, Y., Yan, S., Ji, L., Carbajales-Dale, M., Elyasi, S. N., Marami, H., Tsapekos, P., Liu, H., & Angelidaki, I. (2022). From renewable energy to sustainable protein sources: Advancement, challenges, and future roadmaps. Renewable and Sustainable Energy Reviews, 157. https://doi.org/10.1016/j.rser.2021.112041
Ntinas, G. K., Bantis, F., Koukounaras, A., & Kougias, P. G. (2021). Exploitation of liquid digestate as the sole nutrient source for floating hydroponic cultivation of baby lettuce (Lactuca sativa) in greenhouses. Energies, 14(21). https://doi.org/10.3390/en14217199
Tsapekos, P., Alvarado-Morales, M., Kougias, P. G., Treu, L., & Angelidaki, I. (2021). Enhancing anaerobic digestion of agricultural residues by microaerobic conditions. Biomass Conversion and Biorefinery, 11(6), 2325–2333. https://doi.org/10.1007/s13399-019-00430-4
Tsapekos, P., Kovalovszki, A., Alvarado-Morales, M., Rudatis, A., Kougias, P. G., & Angelidaki, I. (2021). Anaerobic co-digestion of macroalgal biomass with cattle manure under high salinity conditions. Journal of Environmental Chemical Engineering, 9(4). https://doi.org/10.1016/j.jece.2021.105406
Duan, N., Kougias, P. G., Campanaro, S., Treu, L., & Angelidaki, I. (2021). Evolution of the microbial community structure in biogas reactors inoculated with seeds from different origin. Science of the Total Environment, 773. https://doi.org/10.1016/j.scitotenv.2021.144981
Tsapekos, P., Treu, L., Campanaro, S., Centurion, V. B., Zhu, X., Peprah, M., Zhang, Z., Kougias, P. G., & Angelidaki, I. (2021). Pilot-scale biomethanation in a trickle bed reactor: Process performance and microbiome functional reconstruction. Energy Conversion and Management, 244. https://doi.org/10.1016/j.enconman.2021.114491
Ghofrani-Isfahani, P., Tsapekos, P., Peprah, M., Kougias, P., Zhu, X., Kovalovszki, A., Zervas, A., Zha, X., Jacobsen, C. S., & Angelidaki, I. (2021). Ex-situ biogas upgrading in thermophilic up-flow reactors: The effect of different gas diffusers and gas retention times. Bioresource Technology, 340. https://doi.org/10.1016/j.biortech.2021.125694
Gaspari, M., Treu, L., Zhu, X., Palù, M., Angelidaki, I., Campanaro, S., & Kougias, P. G. (2021). Microbial dynamics in biogas digesters treating lipid-rich substrates via genome-centric metagenomics. Science of the Total Environment, 778. https://doi.org/10.1016/j.scitotenv.2021.146296
Louhasakul, Y., Treu, L., Kougias, P. G., Campanaro, S., Cheirsilp, B., & Angelidaki, I. (2021). Valorization of palm oil mill wastewater for integrated production of microbial oil and biogas in a biorefinery approach. Journal of Cleaner Production, 296. https://doi.org/10.1016/j.jclepro.2021.126606
Zhu, X., Campanaro, S., Treu, L., Seshadri, R., Ivanova, N., Kougias, P. G., Kyrpides, N., & Angelidaki, I. (2020). Metabolic dependencies govern microbial syntrophies during methanogenesis in an anaerobic digestion ecosystem. Microbiome, 8(1). https://doi.org/10.1186/s40168-019-0780-9
Louhasakul, Y., Cheirsilp, B., Treu, L., Kougias, P. G., & Angelidaki, I. (2020). Metagenomic insights into bioaugmentation and biovalorization of oily industrial wastes by lipolytic oleaginous yeast Yarrowia lipolytica during successive batch fermentation. Biotechnology and Applied Biochemistry, 67(6), 1020–1029. https://doi.org/10.1002/bab.1878
Campanaro, S., Treu, L., Rodriguez-R, L. M., Kovalovszki, A., Ziels, R. M., Maus, I., Zhu, X., Kougias, P. G., Basile, A., Luo, G., Schlüter, A., Konstantinidis, K. T., & Angelidaki, I. (2020). New insights from the biogas microbiome by comprehensive genome-resolved metagenomics of nearly 1600 species originating from multiple anaerobic digesters. Biotechnology for Biofuels, 13(1). https://doi.org/10.1186/s13068-020-01679-y
Kougias, P. G., Tsapekos, P., Treu, L., Kostoula, M., Campanaro, S., Lyberatos, G., & Angelidaki, I. (2020). Biological CO2 fixation in up-flow reactors via exogenous H2 addition. Journal of Biotechnology, 319, 1–7. https://doi.org/10.1016/j.jbiotec.2020.05.012
Duan, N., Zhang, D., Khoshnevisan, B., Kougias, P. G., Treu, L., Liu, Z., Lin, C., Liu, H., Zhang, Y., & Angelidaki, I. (2020). Human waste anaerobic digestion as a promising low-carbon strategy: Operating performance, microbial dynamics and environmental footprint. Journal of Cleaner Production, 256. https://doi.org/10.1016/j.jclepro.2020.120414
Zhu, X., Campanaro, S., Treu, L., Kougias, P. G., & Angelidaki, I. (2019). Novel ecological insights and functional roles during anaerobic digestion of saccharides unveiled by genome-centric metagenomics. Water Research, 151, 271–279. https://doi.org/10.1016/j.watres.2018.12.041
Angelidaki, I., Xie, L., Luo, G., Zhang, Y., Oechsner, H., Lemmer, A., Munoz, R., & Kougias, P. G. (2019). Biogas upgrading: Current and emerging technologies. Elsevier. https://doi.org/10.1016/B978-0-12-816856-1.00033-6
Sun, H., Kovalovszki, A., Tsapekos, P., Alvarado-Morales, M., Rudatis, A., Wu, S., Dong, R., Kougias, P. G., & Angelidaki, I. (2019). Co-digestion of Laminaria digitata with cattle manure: A unimodel simulation study of both batch and continuous experiments. Bioresource Technology, 276, 361–368. https://doi.org/10.1016/j.biortech.2018.12.110
Porté, H., Kougias, P. G., Alfaro, N., Treu, L., Campanaro, S., & Angelidaki, I. (2019). Process performance and microbial community structure in thermophilic trickling biofilter reactors for biogas upgrading. Science of the Total Environment, 655, 529–538. https://doi.org/10.1016/j.scitotenv.2018.11.289
Tsapekos, P., Khoshnevisan, B., Alvarado-Morales, M., Symeonidis, A., Kougias, P. G., & Angelidaki, I. (2019). Environmental impacts of biogas production from grass: Role of co-digestion and pretreatment at harvesting time. Applied Energy, 252. https://doi.org/10.1016/j.apenergy.2019.113467
Treu, L., Tsapekos, P., Peprah, M., Campanaro, S., Giacomini, A., Corich, V., Kougias, P. G., & Angelidaki, I. (2019). Microbial profiling during anaerobic digestion of cheese whey in reactors operated at different conditions. Bioresource Technology, 275, 375–385. https://doi.org/10.1016/j.biortech.2018.12.084
Tsapekos, P., Alvarado-Morales, M., Kougias, P. G., Konstantopoulos, K., & Angelidaki, I. (2019). Co-digestion of municipal waste biopulp with marine macroalgae focusing on sodium inhibition. Energy Conversion and Management, 180, 931–937. https://doi.org/10.1016/j.enconman.2018.11.048
Angelidaki, I., Treu, L., Tsapekos, P., Luo, G., Campanaro, S., Wenzel, H., & Kougias, P. G. (2018). Biogas upgrading and utilization: Current status and perspectives. Biotechnology Advances, 36(2), 452–466. https://doi.org/10.1016/j.biotechadv.2018.01.011
Kougias, P. G., Campanaro, S., Treu, L., Tsapekos, P., Armani, A., & Angelidaki, I. (2018). Spatial distribution and diverse metabolic functions of lignocellulose-degrading uncultured bacteria as revealed by genomecentric metagenomics. Applied and Environmental Microbiology, 84(18). https://doi.org/10.1128/AEM.01244-18
Kougias, P. G., & Angelidaki, I. (2018). Biogas and its opportunities—A review. Frontiers of Environmental Science and Engineering, 12(3). https://doi.org/10.1007/s11783-018-1037-8
Fontana, A., Kougias, P. G., Treu, L., Kovalovszki, A., Valle, G., Cappa, F., Morelli, L., Angelidaki, I., & Campanaro, S. (2018). Microbial activity response to hydrogen injection in thermophilic anaerobic digesters revealed by genome-centric metatranscriptomics. Microbiome, 6(1). https://doi.org/10.1186/s40168-018-0583-4
Campanaro, S., Treu, L., Kougias, P. G., Luo, G., & Angelidaki, I. (2018). Metagenomic binning reveals the functional roles of core abundant microorganisms in twelve full-scale biogas plants. Water Research, 140, 123–134. https://doi.org/10.1016/j.watres.2018.04.043
Corbellini, V., Kougias, P. G., Treu, L., Bassani, I., Malpei, F., & Angelidaki, I. (2018). Hybrid biogas upgrading in a two-stage thermophilic reactor. Energy Conversion and Management, 168, 1–10. https://doi.org/10.1016/j.enconman.2018.04.074
Omar, B., Abou-Shanab, R., El-Gammal, M., Fotidis, I. A., Kougias, P. G., Zhang, Y., & Angelidaki, I. (2018). Simultaneous biogas upgrading and biochemicals production using anaerobic bacterial mixed cultures. Water Research, 142, 86–95. https://doi.org/10.1016/j.watres.2018.05.049
Treu, L., Campanaro, S., Kougias, P. G., Sartori, C., Bassani, I., & Angelidaki, I. (2018). Hydrogen-fueled microbial pathways in biogas upgrading systems revealed by genome-centric metagenomics. Frontiers in Microbiology, 9(MAY). https://doi.org/10.3389/fmicb.2018.01079
Duan, N., Ran, X., Li, R., Kougias, P. G., Zhang, Y., Lin, C., & Liu, H. (2018). Performance evaluation of mesophilic anaerobic digestion of chicken manure with algal digestate. Energies, 11(7). https://doi.org/10.3390/en11071829
Tsapekos, P., Kougias, P. G., Kuthiala, S., & Angelidaki, I. (2018). Co-digestion and model simulations of source separated municipal organic waste with cattle manure under batch and continuously stirred tank reactors. Energy Conversion and Management, 159, 1–6. https://doi.org/10.1016/j.enconman.2018.01.002
Fontana, A., Campanaro, S., Treu, L., Kougias, P. G., Cappa, F., Morelli, L., & Angelidaki, I. (2018). Performance and genome-centric metagenomics of thermophilic single and two-stage anaerobic digesters treating cheese wastes. Water Research, 134, 181–191. https://doi.org/10.1016/j.watres.2018.02.001
Zhu, X., Treu, L., Kougias, P. G., Campanaro, S., & Angelidaki, I. (2018). Converting mesophilic upflow sludge blanket (UASB) reactors to thermophilic by applying axenic methanogenic culture bioaugmentation. Chemical Engineering Journal, 332, 508–516. https://doi.org/10.1016/j.cej.2017.09.113
Tsapekos, P., Kougias, P. G., & Angelidaki, I. (2018). Mechanical pretreatment for increased biogas production from lignocellulosic biomass; predicting the methane yield from structural plant components. Waste Management, 78, 903–910. https://doi.org/10.1016/j.wasman.2018.07.017
Treu, L., Kougias, P. G., de Diego-Díaz, B., Campanaro, S., Bassani, I., Fernández-Rodríguez, J., & Angelidaki, I. (2018). Two-year microbial adaptation during hydrogen-mediated biogas upgrading process in a serial reactor configuration. Bioresource Technology, 264, 140–147. https://doi.org/10.1016/j.biortech.2018.05.070
Campanaro, S., Treu, L., Kougias, P. G., Zhu, X., & Angelidaki, I. (2018). Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-20414-0
Tsapekos, P., Kougias, P. G., Alvarado-Morales, M., Kovalovszki, A., Corbière, M., & Angelidaki, I. (2018). Energy recovery from wastewater microalgae through anaerobic digestion process: Methane potential, continuous reactor operation and modelling aspects. Biochemical Engineering Journal, 139, 1–7. https://doi.org/10.1016/j.bej.2018.08.004
Bassani, I., Kougias, P. G., Treu, L., Porté, H., Campanaro, S., & Angelidaki, I. (2017). Optimization of hydrogen dispersion in thermophilic up-flow reactors for ex situ biogas upgrading. Bioresource Technology, 234, 310–319. https://doi.org/10.1016/j.biortech.2017.03.055
Lovato, G., Alvarado-Morales, M., Kovalovszki, A., Peprah, M., Kougias, P. G., Rodrigues, J. A. D., & Angelidaki, I. (2017). In-situ biogas upgrading process: Modeling and simulations aspects. Bioresource Technology, 245, 332–341. https://doi.org/10.1016/j.biortech.2017.08.181
Zhu, X., Kougias, P. G., Treu, L., Campanaro, S., & Angelidaki, I. (2017). Microbial community changes in methanogenic granules during the transition from mesophilic to thermophilic conditions. Applied Microbiology and Biotechnology, 101(3), 1313–1322. https://doi.org/10.1007/s00253-016-8028-0
Campanaro, S., Treu, L., Cattani, M., Kougias, P. G., Vendramin, V., Schiavon, S., Tagliapietra, F., Giacomini, A., & Corich, V. (2017). In vitro fermentation of key dietary compounds with rumen fluid: A genome-centric perspective. Science of the Total Environment, 584–585, 683–691. https://doi.org/10.1016/j.scitotenv.2017.01.096
Tsapekos, P., Kougias, P. G., Treu, L., Campanaro, S., & Angelidaki, I. (2017). Process performance and comparative metagenomic analysis during co-digestion of manure and lignocellulosic biomass for biogas production. Applied Energy, 185, 126–135. https://doi.org/10.1016/j.apenergy.2016.10.081
Tsapekos, P., Kougias, P. G., Vasileiou, S. A., Lyberatos, G., & Angelidaki, I. (2017). Effect of micro-aeration and inoculum type on the biodegradation of lignocellulosic substrate. Bioresource Technology, 225, 246–253. https://doi.org/10.1016/j.biortech.2016.11.081
Kougias, P. G., Campanaro, S., Treu, L., Zhu, X., & Angelidaki, I. (2017). A novel archaeal species belonging to Methanoculleus genus identified via de-novo assembly and metagenomic binning process in biogas reactors. Anaerobe, 46, 23–32. https://doi.org/10.1016/j.anaerobe.2017.02.009
Zhu, X., Treu, L., Kougias, P. G., Campanaro, S., & Angelidaki, I. (2017). Characterization of the planktonic microbiome in upflow anaerobic sludge blanket reactors during adaptation of mesophilic methanogenic granules to thermophilic operational conditions. Anaerobe, 46, 69–77. https://doi.org/10.1016/j.anaerobe.2016.12.015
Tsapekos, P., Kougias, P. G., Egelund, H., Larsen, U., Pedersen, J., Trénel, P., & Angelidaki, I. (2017). Mechanical pretreatment at harvesting increases the bioenergy output from marginal land grasses. Renewable Energy, 111, 914–921. https://doi.org/10.1016/j.renene.2017.04.061
Jing, Y., Campanaro, S., Kougias, P., Treu, L., Angelidaki, I., Zhang, S., & Luo, G. (2017). Anaerobic granular sludge for simultaneous biomethanation of synthetic wastewater and CO with focus on the identification of CO-converting microorganisms. Water Research, 126, 19–28. https://doi.org/10.1016/j.watres.2017.09.018
Tsapekos, P., Kougias, P. G., Egelund, H., Larsen, U., Pedersen, J., Trénel, P., & Angelidaki, I. (2017). Improving the energy balance of grass-based anaerobic digestion through combined harvesting and pretreatment. Anaerobe, 46, 131–137. https://doi.org/10.1016/j.anaerobe.2016.12.005
Kougias, P. G., Treu, L., Benavente, D. P., Boe, K., Campanaro, S., & Angelidaki, I. (2017). Ex-situ biogas upgrading and enhancement in different reactor systems. Bioresource Technology, 225, 429–437. https://doi.org/10.1016/j.biortech.2016.11.124
Tsapekos, P., Kougias, P. G., Vasileiou, S. A., Treu, L., Campanaro, S., Lyberatos, G., & Angelidaki, I. (2017). Bioaugmentation with hydrolytic microbes to improve the anaerobic biodegradability of lignocellulosic agricultural residues. Bioresource Technology, 234, 350–359. https://doi.org/10.1016/j.biortech.2017.03.043
Kougias, P. G., Treu, L., Campanaro, S., Zhu, X., & Angelidaki, I. (2016). Dynamic functional characterization and phylogenetic changes due to Long Chain Fatty Acids pulses in biogas reactors. Scientific Reports, 6. https://doi.org/10.1038/srep28810
Treu, L., Kougias, P. G., Campanaro, S., Bassani, I., & Angelidaki, I. (2016). Deeper insight into the structure of the anaerobic digestion microbial community; The biogas microbiome database is expanded with 157 new genomes. Bioresource Technology, 216, 260–266. https://doi.org/10.1016/j.biortech.2016.05.081
Campanaro, S., Treu, L., Kougias, P. G., De Francisci, D., Valle, G., & Angelidaki, I. (2016). Metagenomic analysis and functional characterization of the biogas microbiome using high throughput shotgun sequencing and a novel binning strategy. Biotechnology for Biofuels, 9(1). https://doi.org/10.1186/s13068-016-0441-1
Tsapekos, P., Kougias, P. G., Frison, A., Raga, R., & Angelidaki, I. (2016). Improving methane production from digested manure biofibers by mechanical and thermal alkaline pretreatment. Bioresource Technology, 216, 545–552. https://doi.org/10.1016/j.biortech.2016.05.117
Treu, L., Campanaro, S., Kougias, P. G., Zhu, X., & Angelidaki, I. (2016). Untangling the Effect of Fatty Acid Addition at Species Level Revealed Different Transcriptional Responses of the Biogas Microbial Community Members. Environmental Science and Technology, 50(11), 6079–6090. https://doi.org/10.1021/acs.est.6b00296
Bassani, I., Kougias, P. G., & Angelidaki, I. (2016). In-situ biogas upgrading in thermophilic granular UASB reactor: key factors affecting the hydrogen mass transfer rate. Bioresource Technology, 221, 485–491. https://doi.org/10.1016/j.biortech.2016.09.083
Tsapekos, P., Kougias, P. G., & Angelidaki, I. (2015). Biogas production from ensiled meadow grass; effect of mechanical pretreatments and rapid determination of substrate biodegradability via physicochemical methods. Bioresource Technology, 182, 329–335. https://doi.org/10.1016/j.biortech.2015.02.025
Luo, G., De Francisci, D., Kougias, P. G., Laura, T., Zhu, X., & Angelidaki, I. (2015). New steady-state microbial community compositions and process performances in biogas reactors induced by temperature disturbances. Biotechnology for Biofuels, 8(1). https://doi.org/10.1186/s13068-014-0182-y
Tsapekos, P., Kougias, P. G., & Angelidaki, I. (2015). Anaerobic Mono- and Co-digestion of Mechanically Pretreated Meadow Grass for Biogas Production. Energy and Fuels, 29(7), 4005–4010. https://doi.org/10.1021/ef5027949
Bassani, I., Kougias, P. G., Treu, L., & Angelidaki, I. (2015). Biogas Upgrading via Hydrogenotrophic Methanogenesis in Two-Stage Continuous Stirred Tank Reactors at Mesophilic and Thermophilic Conditions. Environmental Science and Technology, 49(20), 12585–12593. https://doi.org/10.1021/acs.est.5b03451
Kougias, P. G., Boe, K., & Angelidaki, I. (2015). Solutions for Foaming Problems in Biogas Reactors Using Natural Oils or Fatty Acids as Defoamers. Energy and Fuels, 29(7), 4046–4051. https://doi.org/10.1021/ef502808p
Kougias, P. G., Boe, K., Einarsdottir, E. S., & Angelidaki, I. (2015). Counteracting foaming caused by lipids or proteins in biogas reactors using rapeseed oil or oleic acid as antifoaming agents. Water Research, 79, 119–127. https://doi.org/10.1016/j.watres.2015.04.034
Luo, G., De Francisci, D., Kougias, P. G., Laura, T., Zhu, X., & Angelidaki, I. (2015). New Steady-State Microbial Community Compositions and Process Performances in Biogas Reactors Induced by Temperature Disturbances. Apple Academic Press. https://doi.org/10.1201/b18872-10
De Francisci, D., Kougias, P. G., Treu, L., Campanaro, S., & Angelidaki, I. (2015). Microbial diversity and dynamicity of biogas reactors due to radical changes of feedstock composition. Bioresource Technology, 176, 56–64. https://doi.org/10.1016/j.biortech.2014.10.126
Kougias, P. G., Kotsopoulos, T. A., & Martzopoulos, G. G. (2014). Effect of feedstock composition and organic loading rate during the mesophilic co-digestion of olive mill wastewater and swine manure. Renewable Energy, 69, 202–207. https://doi.org/10.1016/j.renene.2014.03.047
Kougias, P. G., Boe, K., Tsapekos, P., & Angelidaki, I. (2014). Foam suppression in overloaded manure-based biogas reactors using antifoaming agents. Bioresource Technology, 153, 198–205. https://doi.org/10.1016/j.biortech.2013.11.083
Kougias, P. G., De Francisci, D., Treu, L., Campanaro, S., & Angelidaki, I. (2014). Microbial analysis in biogas reactors suffering by foaming incidents. Bioresource Technology, 167, 24–32. https://doi.org/10.1016/j.biortech.2014.05.080
Kougias, P. G., Boe, K., O-Thong, S., Kristensen, L. A., & Angelidaki, I. (2014). Anaerobic digestion foaming in full-scale biogas plants: A survey on causes and solutions. Water Science and Technology, 69(4), 889–895. https://doi.org/10.2166/wst.2013.792
Fotidis, I. A., Kougias, P. G., Zaganas, I. D., Kotsopoulos, T. A., & Martzopoulos, G. G. (2014). Inoculum and zeolite synergistic effect on anaerobic digestion of poultry manure. Environmental Technology (United Kingdom), 35(10), 1219–1225. https://doi.org/10.1080/09593330.2013.865083
Kougias, P. G., Fotidis, I. A., Zaganas, I. D., Kotsopoulos, T. A., & Martzopoulos, G. G. (2013). Zeolite and swine inoculum effect on poultry manure biomethanation. International Agrophysics, 27(2), 169–173. https://doi.org/10.2478/v10247-012-0082-y
Kougias, P. G., Tsapekos, P., Boe, K., & Angelidaki, I. (2013). Antifoaming effect of chemical compounds in manure biogas reactors. Water Research, 47(16), 6280–6288. https://doi.org/10.1016/j.watres.2013.07.045
Kougias, P. G., Boe, K., & Angelidaki, I. (2013). Effect of organic loading rate and feedstock composition on foaming in manure-based biogas reactors. Bioresource Technology, 144, 1–7. https://doi.org/10.1016/j.biortech.2013.06.028
Kougias, P. G., Firfiris, V., & Martzopoulos, G. G. (2012). Improvement of a heat pump coefficient of performance used in greenhouses. Acta Horticulturae, 952, 441–448. https://doi.org/10.17660/actahortic.2012.952.55
Firfiris, V., Kougias, P. G., Nikita-Martzopoulou, Ch., & Martzopoulos, G. G. (2012). Performance of a covered closed loop shallow geothermal greenhouse heating system. Acta Horticulturae, 952, 457–462. https://doi.org/10.17660/ActaHortic.2012.952.57
Boe, K., Kougias, P. G., Pacheco, F., O-Thong, S., & Angelidaki, I. (2012). Effect of substrates and intermediate compounds on foaming in manure digestion systems. Water Science and Technology, 66(10), 2146–2154. https://doi.org/10.2166/wst.2012.438
Ntinas, G. K., Kougias, P. G., & Nikita-Martzopoulou, Ch. (2011). Experimental performance of a hybrid solar energy saving system in greenhouses. International Agrophysics, 25(3), 257–264. https://www.scopus.com/inward/record.uri?eid=2-s2.0-80054758214&partnerID=40&md5=067f5791071b384ab84d277570a68b5a
Kougias, P. G., Kotsopoulos, T. A., & Martzopoulos, G. G. (2010). Anaerobic co-digestion of pig waste with olive mill wastewater under various mixing conditions. Fresenius Environmental Bulletin, 19(8 B), 1682–1686. https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957759705&partnerID=40&md5=964ba7a2ee2c87331e82fa709f33363d
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229 publications
As Principal Investigator
BioPro
PI
National
Bioconversion of Biowaste into Single-Cell Proteins within the Framework of the Circular Bioeconomy
Nov 2025 → Nov 2029
FENIX
PI
International
New Life for Biowaste as a sustainable Soil Improver
Jun 2023 → Jul 2027
CRONUS
PI
International
Capture and Reuse Of biogenic gases for Negative-emission - sustainable biofUelS
Dec 2022 → Aug 2026
LIFE CO2toCH4
PI
International
Demonstration of a mobile unit for hybrid energy storage based on CO2 capture and renewable energy sources
Oct 2021 → Jun 2026
